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This book constitutes the refereed proceedings of the 10th International Conference on Data Integration in the Life Sciences, DILS 2014, held in Lisbon, Portugal, in July 2014. The 9 revised full papers and the 5 short papers included in this volume were carefully reviewed and selected from 20 submissions. The papers cover a range of important topics such as data integration platforms and applications; biodiversity data management; ontologies and visualization; linked data and query processing.§