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This book comprises the most significant advances accomplished in enzyme engineering by means of directed evolution and semi-rational approaches. Special attention is paid to the use of directed evolution applied to different biotechnological areas such as fuels and chemicals, biopharmaceuticals and opsin engineering, as well as in emergent approaches linked to this methodology (e.g. computational algorithms, enzyme resurrection, genetic drift, metabolic pathways). The book addresses Professors, graduate students and researchers from both Academia and Industry.