Introduction to Protein Mass Spectrometry

Introduction to Protein Mass Spectrometry, Second Edition, provides a comprehensive overview of this increasingly important, yet complex, analytical technique. This book enables readers to understand how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. This new edition is fully updated to include the latest developments in the field and will feature new content covering recent progress in the areas where there have been the most exciting advances. These include PNNL’s multilevel-PCB-based SLIM realization, SLIM-Agilent QQQ field trials; employment of SLIM-IMS-cryo-IR combination in molecular structure determination; proximity-labelling mass spectrometry, and applications in neuroscience. Citing the most recent and relevant work in the field of biological mass spectrometry, this book is written for researchers and scientists new to the field but is also an ideal resource for those hoping to hone their analytical abilities. Offers up to date, introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitations Provides examples of commonly used MS instruments from a range of key manufacturers, including Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, Waters and PNNL Includes biological applications and exploration of analytical tools and databases for bioinformatics Features definitions, case studies, and recent developments in protein mass spectrometry Includes sections new to this edition on SLIM (Structures for Lossless Ion Manipulation), and spectrometry applications in neuroscience including synaptic biology and Alzheimer’s disease