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Nickel is one of the most environmentally, toxicologically, and industrially significant metals today. This book takes an interdisciplinary approach to addressing aspects related to its chemistry, speciation, environmental distribution, mechanisms of toxicity, and human biomonitoring. It also presents conventional and modern analytical methodologies for the determination of Ni(II), with a special emphasis on molecular fluorescence, solid-state fluorescence (SSF), metallic nanosensors, and strategies compatible with green analytical chemistry. The book integrates theoretical foundations, environmental applications, and experimental contributions developed by the authors in the field of luminescent methodologies applied to the monitoring of nickel in biological and environmental matrices. This book is intended for researchers, faculty, students, and professionals interested in analytical chemistry, environmental toxicology, nanotechnology, and biomedical sciences.