Nucleases

Nucleases are a class of enzymes that catalyze the hydrolysis of nucleic acids (DNA, RNA) in all organisms, including humans. In addition to their important biological role, nucleases have recently emerged as useful tools in laboratory studies, and have led to the development of such fields as recombinant DNA technology, molecular cloning, and genomics. Nawin Mishra's Nucleases, the first comprehensive treatment of the subject, introduces the properties and biological roles of nucleases to newcomers in the field and provides the basis of their possible application to critical aspects of science, commerce, and industry. Covering the structure and biochemical properties of nucleases, Mishra's text further guides the reader to understand how these enzymes can be exploited to make new products for the diagnosis and treatment of disease. Molecular cloning, made possible by the evolving understanding and application of nucleases, may well lead to the identification and characterization of genes responsible for diseases and their possible alleviation by gene therapy and the development of designer drugs. Nucleases is a one-stop, authoritative resource for students of biology and researchers currently practicing in the field. Chapter topics include: * Ribonucleases * Restriction Endonucleases * Topoisomerases * Sugar Non-Specific Nucleases * Molecules That Interact with Nucleases An ideal text and reference work for professionals and students in biochemistry, genetics, and molecular biology, Nucleases promises to be the field's standard-bearer for years to come.