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UV Spectroscopic Method and Method Validation of Methylphenidate
UV Spectroscopic Method and Method Validation of Methylphenidate in Bulk and Marketed Dosage Form Economically. DE
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This UV-spectrophotometric technique is quite simple, accurate, precise,reproducible, and sensitive. The UV method has been developed for quantification of Methylphenidate in tablet formulation. It involves absorbance measurement at 288 nm ( max of Methylphenidate) in Ethanol and distilled water (89:11) ratio. For the UV Spectrophotometric method, linearity was obtained in a concentration range of 1-50µg/ml for methylphenidate and with a regression value of 0.9998. Recovery was found in the range of 98-101%. The standard deviation and % RSD were found to be < 2 %. It showed the high precision of the method, in accordance with ICH guidelines. The method has been successively applied to active pharmaceutical ingredients and was validated according to ICH guidelines. The method is highly accurate, reproducible and economical.
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