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Więcej informacji o Low-Cost Methods for Molecular Characterization of Mutant Plants

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Opis

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

Szczegóły książki

Kategoria Książki po angielsku Mathematics & science Biology, life sciences Botany & plant sciences

• Pełny tytuł: Low-Cost Methods for Molecular Characterization of Mutant Plants
• Podtytuł: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
• Autor: Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
• Język: Angielski
• Oprawa: Miękka
• Liczba stron: 38
• EAN: 9783319386676
• ISBN: 3319386670
• ID: 15194602
• Wydawca: Springer International Publishing AG
• Waga: 97 g
• Wymiary: 235 × 155 × 3 mm
• Data wydania: 17. October 2016

Ulubione w innej kategorii

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